前言目的观察S180小鼠皮下移植瘤组织淋巴管的形态学改变,为进一步研究
肿瘤淋巴管转移机制提供形态学依据。方法将小鼠S180
腹水型瘤细胞接种于昆明小鼠左腋部皮下,20天后切取肿瘤及瘤周组织和对侧正常皮肤,分别用HE染色确认肿瘤,半薄切片筛选淋巴管并观察,超薄切片透射电镜观察淋巴管形态。结果2周以上小鼠腋部可见明显肿块,致癌率达100％。移植瘤中心区液化坏死,无毛细淋巴管。周边区淋巴管检出率明显高于正常侧且管腔大,形态不规则,管壁薄。内皮细胞
肿胀,高尔基体、粗面内质网及溶酶体等细胞器的形态有改变。结论S180移植
肉瘤中心区无淋巴管,瘤周边区淋巴管密度增多,管腔扩张,形态不规则,伴有内皮细胞损伤。
ObjectiveToprovidethemorphologicalbaseforfurtherstudyingthemechanismoftumormetastasis,weobservedmorphologicalchangesoflymphaticvesselsintransplantsubcutaneoussarcomaofS180mice.MethodsS180cellswereinoculatedsubcutaneouslyintoaxillaryfossaeofKMmice.Tumortissues,tumorperipheraltissue,andnormaltissueinoppositesidewereobtainedafter20daysofinoculation.HEstainingwasusedtoobservethedevelopmentandinvasionoftumor,semi-thinsectionwasusedtodetec...
